Journal: Stem Cell Research & Therapy

Article Title: Combining sodium-glucose co-transporter-2 inhibitor with mesenchymal stem cells and brown adipose tissue (BAT) and white adipose tissue (WAT) transplantation to mitigate the progression of diabetic kidney disease: a pre-clinical approach

doi: 10.1186/s13287-025-04358-7

Figure Lengend Snippet: Pancreatic islet analysis. A Representative hematoxylin–eosin (HE) staining for morphological evaluation of pancreatic islets at 14–15 and 18–20 weeks of age. B Pancreatic islet area analysis across experimental groups. (* p < 0.05). C Spearman correlation between islets’ area (µm 2 ) and body weight (g). D Representative images for insulin immunohistochemical analysis. E Quantification of insulin-positive cells per pancreatic islets across groups. ( p < 0.05; * vs. BTBR ob/ob ; # vs. SGLT2i; & vs. SGLT2i + CR; $ vs. SGLT2i + CR + BM-MSCs; continuous line indicated difference between time points). Scale bars represent 100 μm in A and D . Error bars represent mean ± SEM; n = 6 animals

Article Snippet: BTBR ob/ob mice (BTBR.Cg-Lepob/WiscJ; 004824-JAX Laboratories, Bar Harbor, ME, USA), homozygous for leptin gene knockout, serve as a model for T2DM and DKD.

Techniques: Staining, Immunohistochemical staining

Journal: Stem Cell Research & Therapy

Article Title: Combining sodium-glucose co-transporter-2 inhibitor with mesenchymal stem cells and brown adipose tissue (BAT) and white adipose tissue (WAT) transplantation to mitigate the progression of diabetic kidney disease: a pre-clinical approach

doi: 10.1186/s13287-025-04358-7

Figure Lengend Snippet: Immunohistochemical analysis of pancreatic islets. A Representative images of pancreatic sections from experimental groups stained for cleaved caspase-3 at 14–15 and 18–20 weeks of age. B Quantification of cleaved caspase-3-positive cells per pancreatic islets across groups. ( p < 0.05; * vs. BTBR ob/ob ; continuous line indicates difference between time points). Scale bars represent 100 μm in A . Error bars represent mean ± SEM; n = 5 animals

Article Snippet: BTBR ob/ob mice (BTBR.Cg-Lepob/WiscJ; 004824-JAX Laboratories, Bar Harbor, ME, USA), homozygous for leptin gene knockout, serve as a model for T2DM and DKD.

Techniques: Immunohistochemical staining, Staining

Journal: Stem Cell Research & Therapy

Article Title: Combining sodium-glucose co-transporter-2 inhibitor with mesenchymal stem cells and brown adipose tissue (BAT) and white adipose tissue (WAT) transplantation to mitigate the progression of diabetic kidney disease: a pre-clinical approach

doi: 10.1186/s13287-025-04358-7

Figure Lengend Snippet: Gene expression of podocyte markers and components of the RAAS and Ang II/Nox4/TRPC6 axis compared to BTBR wild type. A WT-1. B Nphs1. C Synpo2. D Actn4. E Nox4. F Trpc6. G Ace. H Ace2. I Ace2/Ace ratio. (* p < 0.05). Error bars represent mean ± SEM; n = 5–6 animals

Article Snippet: BTBR ob/ob mice (BTBR.Cg-Lepob/WiscJ; 004824-JAX Laboratories, Bar Harbor, ME, USA), homozygous for leptin gene knockout, serve as a model for T2DM and DKD.

Techniques: Gene Expression

Journal: Inflammation

Article Title: High-Resolution Untargeted Metabolomics Reveals Alternate-Day Fasting May Attenuate Diabetic Kidney Disease Progression in BTBR ob/ob Mice by Affecting the HCA, IPA and Reducing Inflammation

doi: 10.1007/s10753-025-02263-y

Figure Lengend Snippet: Alternate-day fasting alters diabetes in BTBR OB/OB mice. ( A ) Body weight; ( B ) fasting blood glucose; ( C ) Food intake; ( D ) Triglyceride; ( E ) Total cholesterol; ( F ) LDL-C; ( G ) HDL-C; n = 11–15 mice/group.* p < 0.05;** p < 0 .01;*** p < 0 .001; ns p > 0.05; # p < 0.05; ### p < 0 .001.#:WT vs ob/ob; *: ob/ob vs ADF-ob/ob. Statistical analysis was performed using two-way and one-way ANOVA in Prism 10

Article Snippet: BTBR ob/ob mice were obtained from Jackson Laboratory (USA) and housed in the SPF-grade Animal Experimentation Centre at North China University of Science and Technology, Tangshan, China (Approval No. 2022-SY-044).

Techniques:

Journal: Inflammation

Article Title: High-Resolution Untargeted Metabolomics Reveals Alternate-Day Fasting May Attenuate Diabetic Kidney Disease Progression in BTBR ob/ob Mice by Affecting the HCA, IPA and Reducing Inflammation

doi: 10.1007/s10753-025-02263-y

Figure Lengend Snippet: Alternate-day fasting alters diabetic nephropathy in BTBR OB/OB mice. (A) Serum creatinine; (B) Total kidney weight; (C) 24-h proteinuria; (D) . Collagen area proportion. (E) Sirius red staining(× 20). shows high deposition of collagen fibres in the glomerular and tubular interstitium (arrowhead); n = 11–15 mice/group;* p < 0.05, ** p < 0.01 *** p < 0 .001; ns p > 0.05. Statistical analysis was performed using one-way ANOVA in Prism 10

Article Snippet: BTBR ob/ob mice were obtained from Jackson Laboratory (USA) and housed in the SPF-grade Animal Experimentation Centre at North China University of Science and Technology, Tangshan, China (Approval No. 2022-SY-044).

Techniques: Staining

Journal: Inflammation

Article Title: High-Resolution Untargeted Metabolomics Reveals Alternate-Day Fasting May Attenuate Diabetic Kidney Disease Progression in BTBR ob/ob Mice by Affecting the HCA, IPA and Reducing Inflammation

doi: 10.1007/s10753-025-02263-y

Figure Lengend Snippet: Alternate-day fasting intervention improved kidney inflammatory response in BTBR OB/OB mice. (A) Western blot results showed that the expressions of NF-κB P65, IL-6, and MCP-1 were significantly reduced after alternate-day fasting intervention; (B-D) Statistical analysis revealed that alternate-day fasting intervention significantly decreased the expressions of NF-κB P65, IL-6, and MCP-1. n = 11–15 mice/group; * p < 0.05, ** p < 0 .01; *** p < 0 .001; ns p > 0.05. Statistical analysis was performed using one-way ANOVA in Prism 10

Article Snippet: BTBR ob/ob mice were obtained from Jackson Laboratory (USA) and housed in the SPF-grade Animal Experimentation Centre at North China University of Science and Technology, Tangshan, China (Approval No. 2022-SY-044).

Techniques: Western Blot